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Extent of exchange versus normal sialylation
| Asialo fetuin | fetuin | |
|---|---|---|
| CPM | CPM | |
| [9-3H]sialylated asialo fetuin or fetuin (Peak I) | 28265 | 53800 |
| Unused [9-3H]sialyl donor [9-3H]NeuAcα2,3 Galβ 1 ,3 GalNAcβ 1, 3Galα-O-Me (Peak II) | 171120 | 115119 |
| Unused CMP- [9-3H]NeuAc (Peak III) | 50570 | 82086 |
| CMP- [9-3H]NeuAc formed (Peaks I+III) | 78835 | 135886 |
