. Author manuscript; available in PMC: 2012 Nov 8.

Published in final edited form as: Biochemistry. 2011 Oct 13;50(44):9475–9487. doi: 10.1021/bi200301w

Table 2.

Characterization of [9-³H] sialyl labeled glycopeptides obtained following pronase digestion

[9-³H] sialyl labeled Glycoprotein	[9-³H] sialyl label containing Biogel P6 fractions resulting from Pronase digestion
	Major Glycopeptide Fractions	Minor Glycopeptide Fractions
I. HCGβ: 31.4% and 10.2% respectively of the glycoprotein binds ConA- and AAL- whereas 100% of it bound WGA-agarose	Four: the largest size one binding to WGA-agarose and the smallest size not binding to WGA-agarose. The middle two fractions contained both WGA binding, non-binding and loosely binding glycopeptides.	None
II. MN Glycophorin A	One: binding to WGA-agarose and not binding to VVL-agarose	None
III. GlyCAM-1	Two: the large size one binding to WGA-agarose; The small size one not binding to WGA-agarose	None
IV.Fetuin: 100% of glycoprotein binds WGA-agarose	Two: both did not bind to WGA-agarose	One: mostly not binding to WGA-agarose
V. Human placental glycoproteins: 98% bound to WGA-agarose	Two: apparently heterogenous	None
VI. Bovine casein macroglycopeptide: 60% bound to WGA-agarose	One: large in size	One: small in size
VII. Porcine Cowper's gland mucin	One: large in size	Two: smaller in size