Table 1.
Putative target genes of the MtbHLH1 transcription factor
| Relative expression | Relative expression | ||||||
|---|---|---|---|---|---|---|---|
| Mt16KOLIPlus microarrays | qRT-PCR | Boxes in promoter (2) as index | |||||
| Reporter | Affymetrix ID | Annotation | P-value | P35S::MtbHLH1-EAR/Control nodule1 | P35S::MtbHLH1-EAR/Control nodule ± SE1 | E-box | G-box |
| MT014172 | Mtr.40134.1.S1_x_at | Leghaemoglobin 2 | 0.00072 | 0.46 | 0.332 ± 0.123 | 2 | 0 |
| MT009727 | Mtr.9019.1.S1_at | NCR072 | 0.00006 | 0.66 | 0.553 ± 0.195 | nd | nd |
| MT012042 | Mtr.3236.1.S1_at | Late nodulin | 0.00001 | 0.58 | 0.396 ± 0.119 | nd | nd |
| MT003243 | Mtr.13105.1.S1_at | Late nodulin | 0.00010 | 0.58 | 0.297 ± 0.113 | 3 | 1 |
| MT003190 | Msa.955.1.S1_x_at | Nodule-specific GRP-repeat | 0.00004 | 0.66 | 0.576 ± 0.136 | 7 | 0 |
| MT000131 | Mtr.8499.1.S1_at | Asparagine synthase | 0.00004 | 0.59 | 0.29 ± 0.026 | 4 | 1 |
| MT000197 | Mtr.37357.1.S1_at | E-class P450, group I | 0.00002 | 0.81 | 0.562 ± 0.127 | 2 | 0 |
| MT015543 | Mtr.10732.1.S1_at | Wound-responsive family protein | 0.00016 | 0.64 | 0.469 ± 0.101 | nd | nd |
| MT009942 | Mtr.38312.1.S1_at | ABC-2 type transporter | 0.00020 | 0.56 | 0.236 ± 0.066 | nd | nd |
| MT000746 | Mtr.4306.1.S1_s_at | LOB domain protein | 0.00011 | 0.69 | 0.474 ± 0.034 | nd | nd |
| MT007140 | Mtr.8505.1.S1_at | BURP domain protein | 0.00005 | 0.60 | 0.378 ± 0.088 | 1 | 0 |
| MT000263 | Mtr.48601.1.S1_at | BURP domain protein | 0.00007 | 1.99 | 2.383 ± 0.838 | 3 | 0 |
qRT-PCR, quantitative reverse-transcription polymerase chain reaction.
1
Expression ratios calculated between P35S::MtbHLH1-EAR and control nodules either from microarray or qRT-PCR from three independent experiments.
2Number of palindromic E- and G-boxes in the promoter region of the corresponding genes (600 bp for E-boxes, 1500 bp for G-boxes).