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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Biochemistry. 2011 Jul 8;50(32):6855–6866. doi: 10.1021/bi200774w

Figure 3.

Figure 3

Determination of glycan compositions and N-glycosylation types of native bovine ABCA4. (A) An MS/MS spectrum of glycosylated peptide DIFNITDR (residues 501–508) reflects collision-induced dissociation of the attached polysaccharide. (B) List of glycans detected by mass spectrometry for each N-glycosylation site except N98. Abbreviations: Hex, hexose; HexNAc, N-acetylhexosamine; NeuAc, N-acetylneuraminic acid. (C) Staining of a PVDF membrane containing ABCA4 with lectins of various specificities. Abbreviations: ConA, concanavalin A; LCA, Lens culinaris agglutinin; PSA, Pisum sativum agglutinin; UEA-I, Ulex europaeus agglutinin I; WGA, wheat germ agglutinin; Suc. WGA, succinylated WGA; MAL, Maackia amurensis lectin II; RCA120, Ricinus communis agglutinin I; DBA, Dolichos biflorus agglutinin; GSL-I, Griffonia (Bandeiraea) simplicifolia lectin I; SBA, soybean agglutinin; PNA, peanut agglutinin; PHA-E, Phaseolus vulgaris agglutinin E; PHA-L, P. vulgaris agglutinin L. See the Supporting Information for lectin specificities.