Table II.
Parameters of double phenylalanine-substituted mutants
| Mutant name | Without KCNE1 | With KCNE1 | With KCNE3 | |||||||||
| n | I (μA) | V1/2 (mV) | z | n | I (μA) | V1/2 (mV) | z | n | I (μA) | V1/2 (mV) | z | |
| Human KCNQ1 | 6 | 0.5 ± 0.0 | −19.6 ± 0.2 | 2.1 ± 0.1 | 6 | 14.7 ± 1.3 | 30.9 ± 2.5 | 1.6 ± 0.1 | 6 | 1.6 ± 0.2 | NA | NA |
| F127A/F130A | 6 | 0.3 ± 0.0 | −12.1 ± 1.2 | 2.2 ± 0.1 | 6 | 4.9 ± 0.4 | 84.6 ± 1.8 | 2.2 ± 0.1 | 6 | 1.3 ± 0.2 | −14.9 ± 1.6 | 1.4 ± 0.0 |
| F127V/F127V | 6 | 0.5 ± 0.1 | −6.3 ± 1.0 | 2.6 ± 0.1 | 6 | 9.0 ± 0.7 | 81.0 ± 1.8 | 1.9 ± 0.1 | 6 | 1.5 ± 0.2 | −15.5 ± 0.9 | 1.3 ± 0.0 |
| F127L/F130L | 6 | 0.6 ± 0.1 | −6.6 ± 0.4 | 2.3 ± 0.0 | 6 | 10.6 ± 1.1 | 37.8 ± 1.2 | 1.4 ± 0.1 | 6 | 1.0 ± 0.3 | 9.9 ± 2.9 | 1.5 ± 0.1 |
Number of experiments (n), average maximum tail current amplitudes (µA), and parameters deduced from the Boltzmann fitting for the S1 mutants of human KCNQ1. Data were taken from the same batch of oocytes, therefore, the data set for WT human KCNQ1 is different from the data set of Table I. NA, not applicable.