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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Nature. 2010 Jun 24;465(7301):1033–1038. doi: 10.1038/nature09144

Figure 2. PTENP1 3’UTR exerts a tumour suppressive function by acting as a decoy for PTEN-targeting microRNAs.

Figure 2

a–c. PIG/ψ3’UTR-infected DU145 cells show (a) increased PTEN mRNA and protein levels (b) reduced phosho-AKT levels upon EGF stimulation and (c) decreased proliferation rate. d. Growth in semisolid medium of DU145 cells infected with PIG, PIG/ψ3’UTR or PIG/PTEN. e. PTEN mRNA levels 24h after the transfection of pCMV/ψ3’UTR in parental HCT116 or HCT116-DICER−/− cells. Data are normalized using pCMV empty-transfected cells. f. Growth curve of DU145 cells transfected with control siLuc, si-PTEN/PTENP1, si-PTEN or si-PTENP1. g. mRNA levels of PTEN (left) and PTENP1 (right) 24h after the transfection of siLuc (white), si-PTEN/PTENP1 (blue), si-PTEN (black), si-PTENP1 (red). i. Western blot of PTEN 48h after the transfection of the indicated siRNAs. a, c, d, e, f and g. mean ± s.d., n ≥ 3.

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