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. 2011 Oct 31;195(3):485–498. doi: 10.1083/jcb.201103067

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© 2011 Chen and Pollard

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Contractile ring assembly in strains with cofilin mutations. (A) Time course of the accumulation of complete contractile rings in populations of wild-type (adf1⁺), adf1-M2, and adf1-M3 cells with the separation of SPBs at time zero. (B–D) Time-lapse series of negative-contrast fluorescence micrographs showing contractile ring assembly in cells expressing Rlc1p-3GFP and the spindle pole body (SPB) marker Sad1-GFP. Maximum intensity projections were titled 45° to show SPBs and contractile rings and their precursors in B and C. Numbers are times in minutes after SPB separation. Empty arrowheads mark SPBs before they split and filled arrowheads mark separated SPBs. Asterisks mark the completion of contractile rings. Bars, 5 µm. (B) This wild-type cell assembled a complete contractile ring by time +10 min (also see Video 3). (C) This adf1-M2 cell split its SPB at time zero and condensed the nodes into clumps between time −2 to +8 min. During the following 70 min, strands containing myosin-II grew from these clumps and assembled a complete ring, which then constricted (also see Video 4). (D) Two conjoined daughter adf1-M3 cells (DIC image shown on the left) underwent the next round of cytokinesis simultaneously but differed in the time to assemble contractile rings: time +14 min for the bottom cell (filled arrow) and time +28 min for the top cell (empty arrow).