Table 1.

Number of detected protein spots on the 2-DE gel images.

SF Sample Treatment Method (n = 3)	Number of Protein Spots
Untreated	456 ± 33
Acetone Precipitation	320 ± 28+#
2-D Clean-Up Kit	413 ± 31
Acetone and 2-D Clean-Up Kit	278 ± 26*

Comparison between acetone precipitation method and untreated SF sample: + = p < 0.05.

Comparison between acetone precipitation method and 2-D Clean-Up Kit: # = p < 0.05.

Comparison between combined acetone/2-D Clean-Up Kit treated SF sample, and untreated

SF sample, and SF sample treated with acetone precipitation method: * = p < 0.05.

Values expressed as mean ± standard error of means (SEM).