Table 2.

Comparisons of total protein concentrations and their percentages of protein loss after different SF sample treatment methods

SF Sample Treatment	Total Protein Concentration in mg/mL (n = number of samples used)	Percentage of Total Protein Loss (%)
Untreated	21.98 ± 1.66 (n = 6)
Acetone Precipitation	15.79 ± 0.79 (n = 3)+#	22 ± 8
2-D Clean-Up Kit	19.33 ± 0.98 (n = 3)	14 ± 7
Acetone and 2-D Clean-Up Kit	13.41 ± 0.55 (n = 3)‡*	39 ± 5

Comparison between acetone precipitation method and untreated SF sample: + = p < 0.05.

Comparison between acetone precipitation method and 2-D Clean-Up Kit: # = p < 0.05.

Comparison between combined acetone/2-D Clean-Up Kit treated SF sample, and 2-D Clean-Up Kit treated SF sample: ‡ = p < 0.05.

Comparison between combined acetone/2-D Clean-Up Kit treated SF sample, and untreated SF sample, and SF sample treated with acetone precipitation method: * = p < 0.01.

Values expressed as mean ± standard error of means (SEM).