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Assay for cell viability after treating microglial cells and astrocytes with cytokine mixture or LPS + IFNγ. BV-2 and HAPI microglial cells and DITNC astrocytes cultured in 12-well plate were serum starved for 4 h followed by exposure to the three cytokine mixture (3 cyt) or LPS + IFNγ for the respective time indicated. Cell lysates were obtained for MTT assay. Results are mean ± SD from n = 3. **p < 0.01 vs. control (Con).
