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. 2011 Nov;90(5):923–927. doi: 10.1189/jlb.0411182

Figure 4. Inhibitory effects on fMLP-driven PMN leukocyte chemotaxis.

Figure 4.

(A) Human PMN leukoctyes, pretreated with vehicle (PMN leukoctye buffer; –), FXIa (1, 5, 10, 20 μg/ml), PK (20 μg/ml), or TNF-α (10 ng/ml), were placed on fibronectin (5 μg/ml)-coated transwell inserts and allowed to migrate toward the fMLP (10 nΜ)-containing lower chamber for 90 min at 37°C. (B) PMN leukoctyes pretreated with vehicle, FXIa (10 μg/ml), or PK (10 μg/ml) were allowed to migrate toward the IL-8 (100 ng/ml)-containing lower chamber for 90 min at 37°C. The results are presented as mean percentage ± sem of the migratory population for each treatment relative to chemoattractant-driven chemotaxis. *P < 0.05 compared with the migration of vehicle-treated cells to the indicated chemoattractants.