Figure 6. Mucosal adjuvant activity of LT-IIa-B₅.

BALB/c mice or BALB/c(TLR2^−/−) mice were i.n.-immunized at Day 0 and boosted at Days 10 and 20 with OVA (control) or with OVA in combination with LT-IIa-B₅ (OVA+LT-IIa-B₅). (A) Salivary IgA responses. Amounts of IgA are reported as the ratio of anti-OVA IgA to total IgA. (B) Serum IgG responses. Error bars denote 1 sd from the mean for groups of five mice. Similar results to those shown in the figure were obtained in an independent experiment. WT, TLR2-proficient BALB/c mice; TLR2^−/−, TLR2-deficient mice; **, ***statistical differences from the OVA (control) at P < 0.01 and P < 0.001, respectively; ⁺⁺, ⁺⁺⁺statistical difference from the matched control (WT vs. TLR2-deficient) at P < 0.01 and P < 0.001, respectively; NS, OVA versus OVA + LT-IIa-B₅, TLR2^−/− mice.