Figure 5.

(A) Total protein obtained from in vitro translation of muscle and control mRNAs using the rabbit reticulocyte lysate system (Promega Corp.). Lanes: 1, negative control sample with water instead of mRNA; 2, positive control, BMV mRNA; 3, human adult skeletal muscle mRNA; 4, human fetal skeletal muscle mRNA; 5, human adult heart mRNA; and 6, human fetal heart mRNA. (B) Immunoprecipitation of in vitro translated human adult and fetal skeletal muscle proteins. (C) Immunoprecipitation of in vitro translated human adult and fetal heart. Equal amounts of [³⁵S]methionine-labeled proteins were mixed with the appropriate antibody and immunoprecipitated using protein A–Sepharose, and were then run on SDS-polyacrylamide gels. In B and C, the total in vitro translated human heart and muscle proteins were immunoprecipitated with the antibodies denoted by the corresponding lane numbers: 1, preimmune sera; 2, antibody to ZASP; and 3, antibody to myosin. Numbers on the left of the figures indicate rainbow ¹⁴C-methylated protein molecular weight markers, in kD.