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. 1999 Jul 26;146(2):465–476. doi: 10.1083/jcb.146.2.465

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Indirect immunofluorescence of undifferentiated human myoblasts (A) and differentiated human myotubes (B). Preimmune sera and ZASP pAb were used at a dilution of 1/50; myosin mAb (MF 20) was used at 1/100 dilution. FITC-conjugated anti-mouse immunoglobulin (Sigma Chemical Co.) was used as the second antibody. (C) Indirect immunofluorescence of skeletal muscle tissue sections using mouse pAb to ZASP (1/30 dilution) and rabbit antiactin antibody (1/40). FITC-labeled goat anti–rabbit Ig (green) and TRITC-labeled goat anti–mouse Ig (red) were used as second antibodies. Bars, 10 μm.