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. 1999 Jul 26;146(2):465–476. doi: 10.1083/jcb.146.2.465

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of ZASP in human skeletal muscle by immunoelectron microscopy. Immunoelectron microscopy with ZASP pAb as the primary antibody and anti-mouse IgG whole molecule conjugated with 5-nm gold particles (Sigma Chemical Co.; G7527) as the secondary antibody. (A) Low magnification of a section of skeletal muscle. The gold particles were detected in the Z-band. (B) Higher magnification of the same image, rotated 90°. The ZASP protein is seen throughout the Z-band. Bars, 0.1 μm.