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. 1999 Jul 26;146(2):273–284. doi: 10.1083/jcb.146.2.273

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of p180 without its ribosome binding domain produces extensive smooth membrane proliferation different from karmellae. Cells were transfected with pYEX-BX containing the ΔNT version of p180. Transformants were selected and grown in Cu²⁺-containing medium for 5 h before preparation for electron microscopy. Visible are parallel arrays of smooth membranes. Note the exclusion of cytoplasmic ribosomes from the areas where membranes are evident, and their high density in membrane-free zones. Bars, 200 nm.