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. 1999 Jul 26;146(2):273–284. doi: 10.1083/jcb.146.2.273

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of the NH₂-terminal half of p180 induces extensive, closely spaced rough membranes. Cells were transfected with pYEX-BX containing the ΔCT version of p180. Transformants were selected and grown in Cu²⁺-containing medium for 5 h before preparation for electron microscopy. Visible are closely opposed rough membranes with a lower density of ribosomes in membrane-free zones (compared with cells shown in Fig. 4). Bars, 200 nm.