Table 1.
Quantification of Northern Analysis of Gene Expression Induced by p180 Constructs
Expression of: | Northern Analysis Probed with: | |||||||
---|---|---|---|---|---|---|---|---|
Gene: | PYK1 | KAR2 | SEC61 | SEC72 | OST1 | SAC1 | GDA1 | SEC1 |
Location: | Cytosol | RER/L | RER/M | RER/M | RER/M | RER/G | Golgi | SV |
Vector-only | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
HMG1 | 1.0 | 3.5 | 1.3 | 0.9 | 0.5 | 1.7 | 0.8 | 1.4 |
HMG2 | 1.0 | 3.7 | 1.3 | 0.5 | 0.3 | 1.1 | 0.6 | 0.8 |
p180 Full-L | 1.0 | 3.2 | 10.3 | 3.5 | 5.3 | 3.9 | 2.4 | 1.7 |
p180 ΔNT | 1.0 | 2.2 | 2.9 | 2.7 | 4.1 | 2.7 | 1.8 | 1.2 |
p180 ΔCT | 1.0 | 3.8 | 14.9 | 3.6 | 6.9 | 9.1 | 5.0 | 6.1 |