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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Immunobiology. 2011 May 14;216(10):1085–1093. doi: 10.1016/j.imbio.2011.05.007

Figure 5. DCs from MLN of aged mice are less efficient to induce TGF-β production and CD4+LAP+ regulatory T cells when co-cultured with OVA-TCR transgenic T cells.

Figure 5

Pooled DCs were obtained from mesenteric lymph nodes and spleen of 5 non-manipulated mice (2 and 12-month-old). Pools of T cells were obtained from spleen of 5 non-manipulated DO11.10 mice (2-month-old). DCs from 2- or 12-month-old mice from MLN or spleen were co-cultured with T cells from 2-month-old DO11.10 mice and stimulated with either medium or OVA (1mg/ml) for 72h at 37°C. Supernatants were collected for cytokine assays. IFN-γ (A), IL-4 (B), IL-10 levels (C) and TGF-β (D) were measured by ELISA, frequency of CD25+LAP+ cells (E), gated in CD4+ T cells, assessed by flow cytometry.