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. 2010 Jan 7;2(1):24–53. doi: 10.3390/toxins2010024

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© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) Diagram for the preparation of an immuno-affinity matrix for BoNT enrichment. Protein A beads are coupled to anti-BoNT antibodies and crosslinked via the Fc domain with disuccinimidyl suberate. Non-crosslinked antibodies are washed out. (B) Immobilized BoNT/A cleaves FRET substrate restoring fluorescence. Reprinted from [112], Copyright © 2008 the authors.