Skip to main content
. Author manuscript; available in PMC: 2012 Oct 20.
Published in final edited form as: Neuron. 2011 Oct 20;72(2):300–315. doi: 10.1016/j.neuron.2011.09.001

Figure 5. Molecular Dissociation of OPHN1’s Role in Regulating Basal Synaptic Strength and mGluR-LTD.

Figure 5

(A–C) Normalized EPSC amplitudes plotted against time from pairs of uninfected and OPHN1#2+OPHN1GAP (A), OPHN1#2+OPHN1Hom (B), or OPHN1#2+OPHN1Endo (C) infected neurons during baseline and after LTD induction (100 µM DHPG, 5 min). Top panels: Representative traces of AMPAR-EPSCs before and after DHPG induction for control and infected neurons. Scale bars, 30 ms and 20 pA. Bottom left panels: EPSC amplitudes normalized to the baseline of uninfected neurons (Unscaled). Bottom right panels: EPSC amplitudes normalized to the baseline responses of each cell (Scaled).

(D) Baseline (DHPG -) and 30–35 min post DHPG (DHPG +) data are summarized. Baseline AMPAR currents and AMPAR currents 30–35 min after DHPG application are normalized to baseline AMPAR currents of adjacent uninfected neurons for each indicated condition. Data are shown as mean ± SEM. *p < 0.005, paired t-test. See Supplemental Data for quantifications.