Fig. 5.

Leptin signaling in kisspeptin-immunopositive neurons in response to an acute injection of leptin (5 mg/kg, ip). Phosphorylated STAT3 (A and B), pSTAT5 (C and D), or pS6 (E and F) were not colocalized with kisspeptin-expressing neurons in the RP3V of adult female mice. The dotted square on the left is magnified on the right. Arrows indicate a lack of pSTAT3/5 or pS6 immunoreactivity in kisspeptin-expressing cells. Arrowheads indicate pSTAT3/5 or pS6 (green) immunoreactivity in unidentified cells.