Skip to main content
. 2011 Nov;27(11):1213–1222. doi: 10.1089/aid.2010.0321

FIG. 6.

FIG. 6.

Reactivity of Env proteins with CD4 and CCR5 receptors. (A) Env-CD4 binding. A 96-well plate coated with OKT4 antibody and then with soluble CD4 was incubated with virions pseudotyped with Env constructs. After extensive washes the bound virions were detected by anti-SIV peroxidase-conjugated antibodies. Results represent percentage of bound virions compared to that observed for wild-type full-length Env samples (100%). The results were analyzed by the Microsoft Excel program and the p values obtained for statistical comparison between samples. (B) Blocking of infection by SIV Env pseudotyped virions by mAb 2D7. A virus entry assay based on single-cycle infection as described in Materials and Methods was used. The blocking of infection was measured by comparison of the number of blue cells in wells with antibody-treated cells to the number in wells with untreated cells. Results are shown with the standard deviation (n=3).