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. 2011 Nov 2;6(11):e25890. doi: 10.1371/journal.pone.0025890

Figure 2. painless mutant males exhibited a male–male courtship behavior.

Figure 2

(A) The average Chaining index (ChI) of 8–10 males that displayed spontaneous chaining behavior during a 10 min period in the morning (ZT0.5–ZT1.5). For both WT and painGal4males, more than six groups of males were analyzed. P<0.01 vs. the WT (Kruskal-Wallis test). (B) Average ChI of males recovered from the indicated anesthesia (top). Three different anesthetics were used in four different doses (indicated in the boxes above the traces). For each trace, 12 groups of males were observed every 5 min with a 15 min interval. (C) Average time spent by eight WT males on courting (left) and average time of courtship initiation (middle), before and after the indicated anesthesia treatments (grey arrows). Average time spent by the WT males on each courtship bout after anesthesia treatment (right). Values shown are means ± SEM.