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. 2011 Nov 2;6(11):e26370. doi: 10.1371/journal.pone.0026370

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Sachs et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Relative growth effects (panels A, B) and nodulation rates (C, D) are shown for experimentally evolved strains #4 and #35 compared to their ancestors. Relative growth effects were analyzed by subtracting the size-matched control plant shoot biomass from the shoot biomass of each inoculated plant. Nodulation rates are the number of nodules per inoculated plant. Strains #4 and #35 are shown because they exhibited the greatest evolutionary increase in in vitro fitness as well as the largest reduction in symbiotic quality.