Table 1. Phenotypic and genotypic analysis of 75 Bradyrhizobium strains.
| Strain Code1 | Nod?2 | PCR Assay3 | Strain Code1 | Nod?2 | PCR Assay3 | ||||
| nifD | nifH | nodDA | nifD | nifH | nodDA | ||||
| 1_05LoS24R3.29 | N | 0 | 0 | 0 | 39_05LoS16R10.36 | Y | 1 | 1 | 1 |
| 2_05LoS24R3.28 | Y | 1 | 0 | 1 | 40_05LoS16R3.25 | N | 0 | 0 | 0 |
| 3_05LoS23R5.4 | Y | 1 | 1 | 1 | 41_05LoS16R1.17 | N | 0 | 0 | 0 |
| 4_05LoS21R5.36 | Y | 1 | 1 | 1 | 42_05LoS16R12.38 | Y | 1 | 1 | 1 |
| 5_05LoS21R1.15 | N | 0 | 0 | 0 | 43_05LoS16R8.27 | N | 0 | 0 | 0 |
| 6_05LoW31R2.26 | N | 0 | 0 | 0 | 44_05LoS16R2.18 | N | 0 | 0 | 0 |
| 7_05LoS25.1 | Y | 1 | 1 | 1 | 45_05LoS16R1.16 | Y | 1 | 0 | 1 |
| 8_05LoS21R5.37 | N | 0 | 0 | 0 | 46_05LoS16R2.19 | Y | 1 | 0 | 1 |
| 9_05LoM26R1.46 | N | 0 | 0 | 0 | 47_05LoS23R3.47 | N | 0 | 0 | 0 |
| 10_05LoS25R2.13 | Y | 1 | 1 | 1 | 48_05LoS25R5.28 | N | 0 | 0 | 0 |
| 11_05LoS23.7 | Y | 1 | 1 | 1 | 49_05LoS23R7.12 | Y | 1 | 0 | 1 |
| 12_05LoH15.1 | Y | 1 | 1 | 1 | 50_05LoS23R5.3 | Y | 1 | 0 | 1 |
| 13_05LoM26.3 | Y | 1 | 1 | 1 | 51_05LoH15R8.9 | Y | 1 | 0 | 1 |
| 14_05LoM26.5 | Y | 1 | 1 | 1 | 52_05LoH15R2.48 | N | 0 | 0 | 0 |
| 15_05LoS21R6.41 | N | 0 | 0 | 0 | 53_05LoM26R4.10 | N | 0 | 0 | 0 |
| 16_05LoS21.3 | Y | 1 | 1 | 1 | 54_05LoM26R2.50 | N | 0 | 0 | 0 |
| 17_05LoS21R1.14 | N | 0 | 0 | 0 | 55_05LoH15R5.50 | Y | 1 | 0 | 1 |
| 18_05LoS21R6.43 | Y | 1 | 1 | 1 | 56_05LoS22.5 | Y | 1 | 0 | 1 |
| 19_05LoS21R3.26 | N | 0 | 0 | 0 | 57_05LoS23R3.49 | N | 0 | 0 | 0 |
| 20_05LoS21R3.24 | N | 0 | 0 | 0 | 58_05LoHR2.45 | N | 0 | 0 | 0 |
| 21_05LoS21R3.23 | N | 0 | 0 | 0 | 59_05LoH15R8.7 | N | 0 | 0 | 0 |
| 22_05LoS4.2 | Y | 1 | 1 | 1 | 60_05LoS23R4.50 | Y | 1 | 1 | 1 |
| 23_05LoS7.4 | Y | 1 | 1 | 1 | 61_05LoS23R3.45 | N | 0 | 0 | 0 |
| 24_05LoS14.1 | Y | 1 | 1 | 1 | 62_05LoS24R8.1 | N | 0 | 0 | 0 |
| 25_05LoS21.4 | Y | 1 | 1 | 1 | 63_05LoS24R1.19 | N | 0 | 0 | 0 |
| 26_05LoS21R5.38 | N | 0 | 0 | 0 | 64_05LoS24R2.25 | N | 0 | 0 | 0 |
| 27_05LoS21R2.18 | N | 0 | 0 | 0 | 65_05LoS24R2.27 | N | 0 | 0 | 0 |
| 28_05LoS21R6.42 | N | 0 | 0 | 0 | 66_05LoS24R3.31 | N | 0 | 0 | 0 |
| 29_05LoS22R1.2 | N | 0 | 0 | 0 | 67_05LoS24R3.32 | N | 0 | 0 | 0 |
| 30_05LoS22.10 | Y | 1 | 1 | 1 | 68_05LoS24R5.42 | N | 0 | 0 | 0 |
| 31_05LoS22R3.12 | Y | 1 | 1 | 1 | 69_05LoS24R5.41 | N | 0 | 0 | 0 |
| 32_05LoS22R5.22 | Y | 1 | 1 | 1 | 70_05LoS25.2 | Y | 0 | 0 | 1 |
| 33_05LoS22R8.36 | Y | 1 | 1 | 1 | 71_05LoS25.4 | Y | 0 | 0 | 1 |
| 34_05LoS22R7.31 | Y | 1 | 1 | 1 | 72_05LoS25R2.15 | N | 0 | 0 | 0 |
| 35_05LoS3.1 | Y | 1 | 1 | 1 | 73_05LoS25R3.19 | N | 0 | 0 | 0 |
| 36_05LoS22.1 | Y | 1 | 1 | 1 | 74_05LoS25R5.29 | N | 0 | 0 | 0 |
| 37_05LoS16R10.32 | Y | 1 | 1 | 1 | 75_05LoS25R5.30 | N | 0 | 0 | 0 |
| 38_05LoS3.3 | Y | 1 | 1 | 1 | |||||
1
Strain codes include year of isolation (05 = 2005), host species (LoA = Lotus angustissimus, LoM = L. micranthus, LoH = L. heermannii, LoS = L. strigosus), plant number, and nodule or root-surface number (the latter with R followed by root and isolate number).
2
Results of greenhouse nodulation assays in which each Bradyrhizobium isolate was tested on 5–8 inoculated seedlings to examine nodulation capability.
3
Results of PCR amplification assays in which we attempted to amplify each of three symbiosis loci (nifD, nifH, nodDA). Unsuccessful reactions were repeated thrice to confirm lack of amplification.