Table 1. Sequencing quality evaluation of the cDNA samples of P. pastoris GS115 cultured under SMG and NG conditions.
Tags sequenced | Stationary (SMG) | Stationary (NG) | Exponential (SMG) | Exponential (NG) | |
Number of total tags | Total tags | 3748512 | 3932994 | 3748455 | 5200265 |
Distinct tags | 87368 | 61891 | 87367 | 82681 | |
Number of clean tags | Total tags | 3716288 | 3262032 | 3149116 | 5077915 |
Distinct tags | 76356 | 70016 | 106337 | 85159 | |
Number of tags matched to gene | Total tags | 568040 | 800087 | 568043 | 450031 |
Distribution of total tags | 15.28% | 24.53% | 15.16% | 10.06% | |
Distinct tags | 12058 | 18082 | 16894 | 10998 | |
Distribution of distinct tags | 12.22% | 18.65% | 15.95% | 10.04% | |
Number of tags matched to unique gene | Total tags | 1986486 | 1446734 | 1987115 | 659187 |
Distribution of total tags | 53.45% | 44.36% | 52.32% | 12.98% | |
Distinct tags | 14514 | 10938 | 15143 | 10366 | |
Distribution of distinct tags | 25.75% | 24.55% | 17.55% | 20.95% | |
Number of unknown tags | Total tags | 648151 | 571776 | 665218 | 731177 |
Distribution of total tags | 17.44% | 17.53% | 17.76% | 22.76% | |
Distinct tags | 30512 | 23829 | 47579 | 25104 | |
Distribution of distinct tags | 54.14% | 59.78% | 55.14% | 55.55% |