Figure 3. Kinesin^CaaX is farnesylated in T. brucei.

Radioactivity (CPM: counts per minute) found in HA-antibody immunoprecipitates from induced HA-Kinesin^CaaX (Kinesin+tet), induced HA-Kinesin^{CVIM deletion} (CVIM- +tet), and control non-transfected cells (Control+tet) extracts from bloodstream form cells. Cells were treated with tetracycline, simvastatin and incubated with ³H mevalonolactone for 48 hours. Radioactivity above background is found only in the induced HA-Kinesin^CaaX –immunoprecipitates. Whole cell lysates pre-IP were compared using a scintillaton counter to ensure equal lysate concentrations per reaction. Statistical differences were assessed with a Student T Test.