Figure 1. Sequence alignment of the present full-length HIV-1 Nef (NefRP14) with NL4-3.
NL4-3 and RP14 sequences are shown in green and black color respectively. The main differences between the sequences involve the insertion of 9 residues after Arg20 and the deletion of the CAWLEAQ motif containing residues 55-61 in the NL4-3 sequence. The conserved Asp123 of the earlier NL4-3 sequence corresponds to Asp125 in the present structure. The XR motif involving residues 105–106 in the earlier NL4-3 sequence corresponds to residues 107–108 in the present structure. The PXXP motif in the present structure is from residues 74–80 while the 30-residue disordered loop distal to this motif ranges from residues 151–180.
