Figure 3. Receiver operating characteristic (ROC) curves of sense strand methylation (solid line, panel A) or antisense strand methylation (dashed line, panel A) of the APC gene as a marker to discriminate HCC (n = 58) from all non-HCC liver tissues including normal (n = 6), hepatitis (n = 39), cirrhosis (n = 41), and matched adjacent non-HCC (n = 58).

The amount of methylated DNA was the average of two duplicate MSP assays as detailed in Materials and Methods. The area under the curve of each ROC curve and statistical analyses are shown in panel B.