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. 2011 Nov 2;6(11):e26974. doi: 10.1371/journal.pone.0026974

Table 5. Strains used in this study.

Strain characteristics selective marker reference
E. coli HB101 F–, thi-1, hsdS20 (rB , mB ), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1. Promega
T7 lysY Express MiniF, lysY (CamR) / fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS )2 [dcm] R(zgb-210::Tn10--TetS ) endA1 Δ(mcrC-mrr)114::IS10 chloramphenicol NEB
VK1 E. coli T7 lysY Express transformed with pET28c::msrA kanamycin This study
VK2 E. coli T7 lysY Express transformed with pET28c::msrB kanamycin This study
S. Typhimurium wild type - ATCC 14028
SHO11 ΔmsrA chloramphenicol This study
SHO18 ΔmsrAΔmsrB chloramphenicol, kanamycin This study
SHO19 ΔmsrB kanamycin This study
SHO35 ΔmsrA transformed with pBAD30::msrA chloramphenicol, ampicillin This study
SHO36 ΔmsrAΔmsrB transformed with pBAD30::msrA chloramphenicol, kanamycin, ampicillin This study
LD24 ΔmetCΔmsrB kanamycin This study
LD40 ΔmetCΔmsrC chloramphenicol This study
LD41 ΔmetCΔmsrBΔmsrC chloramphenicol, kanamycin This study
LD39 ΔmsrC chloramphenicol This study
LD49 ΔmsrBΔmsrC chloramphenicol This study
LD55 ΔmsrC transformed with pBAD30::msrC chloramphenicol, ampicillin This study