Table 6. Primers used in this study.

Primer	Sequence (5′→3′)	Purpose
msrA1	ATG CGT TAC CAG GAC GCA ACA CCC CGA TGC GAT CGC CAC TCT GCG TAG GCT GGA GCT GCT TC	Deletion of msrA
msrA2	GCG ATG GTC TCC GGC GGC GGT CAT TGC CGA CTG AAA GCG CTC GCG TAT GAA TAT CCT CCT TAG	Deletion of msrA
msrA ST fwd	CTGGTTACTCAAGCAGATGCG	Confirmation of msrA deletion
msrA ST rvs	CAGGGCTAGTATAGCGTAAGC	Confirmation of msrA deletion
msrB1	GTG TTA ATG TTT TGT TAG AAT CGG TCA GGC AAT GTG AGC ACG GTA GGC TGG AGC TGC TTC	Deletion of msrB
msrB2	GTC CGC TCC TGT GGA ATA ATT TGC TGA ATC GTT TTT TCA GCC TAT GAA TAT CCT CCT TAG	Deletion of msrB
msrB test 1	GATTCGTGAGCCGCCATTTC	Confirmation of msrB deletion
msrB test 2	GATACACTTCCGGCGTCATG	Confirmation of msrB deletion
metC_For	CAT GCT AGT TTA GAC ATC CAG ACG GTTAAA ATC AGG AAA CGC AAC GTA GGC TGG AGC TGC TTC	Deletion of metC
metC_Rev	CAG ACT TTT CCA CGG AAA TTG TCT GCA TAT ATG TCC ATC CCC GCC TAT GAA TAT CCT CCT TAG	Deletion of metC
k metC_For	CGT CGC CAG GGT GCA GAT GG	Confirmation of metC deletion
k metC_Rev	GCC TTG ATC CCG GAC GCA AC	Confirmation of metC deletion
msrC_For	GAC CAC GAA GTG ATT ATA TAA TGA GCA AAA CAG AAC TAT ACG CGG GTA GGC TGG AGC TGC TTC	Deletion msrC
msrC_Rev	CAA ACG CCG TGC TAT CTA TAT CCA GCA CGC CGA TAA TCC GTT CGC TAT GAA TAT CCT CCT TAG	Deletion msrC
k msrC_For	GAG CAA GAA CGC ATT TAA TGC	Confirmation of msrC deletion
k msrC_Rev	GCG TAC GCA GGC CGT GT TC	Confirmation of msrC deletion
msrA1_HindIII	ATA TAT AAG CTT CTC AGG CAC AGT AAG CTA AC	Complementation with msrA
msrA2_HindIII	ATA TAT AAG CTT TTC AAC TCC GAC AAG TTC CC	Complementation with msrA
msrC_ HindIII	ATA TAT AAG CTT CTG CGT CAT TTG CCA GAT GC	Complementation with msrC
msrC_ HindIII	ATA TAT AAG CTT TCG GCC AGA AAC GCG ATA AC	Complementation with msrC
msrA_F_NheI	ATA TAT GCT AGC ATG AGT TTA TTT GAT AAA AAA CAT CTG	Overexpression MsrA
msrA_R_BamHI	ATA TAT GGA TCC TCA CGC GTC AGG CGG CAG G	Overexpression MsrA
msrB_F_NheI	ATA TAT GCT AGC GTG AGC ACG TTT AAA GTG AGA TG	Overexpression MsrB
msrB_R_BamHI	ATA TAT GGA TCC TCA GCC TTT CAG TTG ATC GCC G	Overexpression MsrB