Figure 1. UGGT activity in C. elegans.

C. elegans (A) and rat liver (B) microsomal proteins were incubated in a mixture that contained 5 mM Tris-maleate buffer, pH 7.5, 10 mM CaCI₂, 0.6% Triton X-100, 5 mM NMDNJ and 3 µCi UDP-[¹⁴C]Glc, at 24°C (A) and 37°C (B) for 60 min. Glycans obtained by Endo H treatment were subjected to paper chromatography with solvent A. Standards G1M9: Glc₁Man₉GlcNAc; G1M8: Glc₁Man₈GlcNAc and G1M7: Glc₁Man₇GlcNAc.