Figure 7. uggt-1 expression is regulated by the ire-1 arm of the unfolded response pathway.

Total RNA from young adults, wild type, ire-1, atf-6 and pek-1 worms, treated and not treated with 5 µg/ml TN was extracted and the levels of mRNA coding for uggt-1, uggt-2 and hsp-4 were analyzed by real time PCR using ama-1 as reference gene. Gene expression level represents RNA expression in TN treated worms/RNA expression in untreated worms. The value obtained for untreated samples was considered as one. The values shown are the mean of three independent experiments. Error bars represent standard deviations * indicates significant differences.