Figure 9. CeUGGT-1 depletion causes a reduced lifespan and that of CeUGGT-1 and CeUGGT-2 a delayed development.

F2 uggt-1(RNAi) uggt-2(RNAi) and control gfp (RNAi) worms were seeded on RNAi plates and monitored for survival at 24 h intervals for the next 16 days (A). The survival analysis was performed using the Kaplan-Meier method and the survival curves were compared using the logarithmic rank test. Development to progressive larval stages (B). Gravid hermaphrodites were transferred to RNAi plates for 6 h to lay eggs and the developmental stages of the worms were analyzed for 5 days at intervals of 24 h and the percentages of L1, L2/L3, L4 and adults were determined. These studies were performed for three independent cohorts (n>60) and the results are representative of triplicate experiments (A) and the mean of the three experiments (B).