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. 2011 Nov 2;6(11):e27092. doi: 10.1371/journal.pone.0027092

Figure 4. Coomassie Blue stained SDS-PAGE analysis for the purification of recombinant human PCNA after FPLC chromatography on Mono P column.

Figure 4

Wild-type PCNA from the expression of one liter of DH5-α cells was purified on Q-sepharose column. Collected peak fractions were further purified on 4-ml Mono P HR 5/20 column. The starting material dialyzed in TGEED (Bc), flow-through (Ft), wash (W), and eluted fractions (45-75) were analyzed on 12% SDS-PAGE followed by Coomassie Blue staining. Protein marker is indicated in kDa by “M”. the position of PCNA is indicated by an arrow.