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. 2011 Nov 2;6(11):e27092. doi: 10.1371/journal.pone.0027092

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Hela cells were fixed, permeabilized, and co-stained for p50 and PCNA using indirect immunofluorescence in which an anti-p50 rabbit polyclonal antibody was used for p50 while an anti-PCNA mouse monoclonal antibody PC-10 was used for PCNA. A: Rhodamine-X-conjugated anti-rabbit IgG secondary antibody was used for p50 (red). B: Alexafluor488-conjugated anti-mouse IgG secondary antibody was used for PCNA (green). C: DNA was counterstained with DAPI immunofluorescence (blue). D: Merger for p50 (panel A) and DAPI (panel C). E: Merger for p50 (panel A) and PCNA (panel B). F: Merger for p50 (panel A), PCNA (panel B), and DAPI (panel C). Cells were analyzed and photographed with an Axiovert 200 M fluorescence microscope (Zeiss).