Table 1. List of qRT-PCR primers.

Gene name	Forward primer	Reverse primer	Acc no.
β-catenin	5′-GCCAAGTGGGTGGCATAGA-3′	5′-TCCCTGTCACCAGCACGAA-3′	NM_053357.2
Dkk-1	5′-CTGTCTGCCTCCGATCATCA-3′	5′-CAGAAATGTCTTGCACAACACA-3′	NM_001106350.1
Dkk-2	5′-GCTCGCGGGCCAAAC-3′	5′-CAACTCCATCAAGTCC-3′	NM_001106472.1
Dkk-3	5′-CAGCTGTGACATCCAGACAGAAG-3′	5′-GCACCTGAAACTGTCATCTGAGA-3′	NM_138519.1
Dkk-4	5′-AGGCCTCTGTGGGCAACTT-3′	5′-CTAAGGCTGGGCTGCTGAGT-3′	NM_001109332.1
GSK-3β	5′-TCTGGCCACCATCCTTATCC-3′	5′-TTGCAGGCGGTGAAGCA-3′	NM_032080.1
sFRP-1	5′-CTGCCACCAGCTGGACAAC-3′	5′-ACCTTGCGCCCCATGA-3′	XM_224987.4
sFRP-2	5′-CGTGAAACGGTGGCAGAAG-3′	5′-CGGATGCTGCGGGAGAT-3′	NM_001100700.1
sFRP-3	5′-CTACCCTGGAACATGACCAAGAT-3′	5′-TGGCGTTAGCCTGGGTACTG-3′	NM_001100527.1
TCF-1	5′-GCTGCTTCAGGGCTAAGATTGT-3′	5′-TGTGACCTTGGCATGAGTTACG-3′	NM_012669.1
Wnt6	5̀-GCGGTCACTCAGGCCTGTT-3′	5′-GGGTGCCTGACAACCACACT-3′	NM_001108226.1
Wnt8	5′-CCTGGGAGCGGTGGAACT-3′	5′-CCTGGTGTGGGTTGAAAACTG-3′	NM_001106155.1
Wnt10b	5 -CCTCAAGCGCGGTTTCC-3′	5′-CAGCAGCCAGCATGGAGAA-3′	NM_001108111.1
Wnt15	5′-CACCCATGTGGGCATCAA-3′	5′-CCATGACACTTGCAGGTTGTTC-3′	NM_001107055.1
Wif1	5′-TGCGGTGCCCATGGA-3′	5′-CTGCCACGAACCCA-3′	NM_053738.1

Primers used for qRT-PCR analysis of the genes assessed here, including the gene symbol, primer sequence (forward and reverse sequence respectively) and GenBank accession number. The primers used to assess the expression of Wnt ligands and inhibitors not included in the list were obtained elsewhere [47].