Table 1. Regions of the HIV genome analyzed using the high resolution melting (HRM) diversity assay.

Region analyzeda	Corresponding region in HXB2a	Sequences of primers used to produce amplicons for HRM diversity analysisb	Amplicon size (bp)
GAG1	1998 – 2097	Forward: 5′- AAATTGCAGGGCCCCTAGGAA	100
		Reverse: 5′- TTTCCCTAAAAAATTAGCCTGTCT
GAG2	2068 – 2278	Forward: 5′- ACTGAGAGACAGGCTAATTTTTTAG	211
		Reverse: 5′- GGTCGTTGCCAAAGAGTGATTTG
POL	2373 – 2597	Forward: 5′- AAATGGAAACCAAAAATGATAG	225
		Reverse: 5′- CATTCCTGGCTTTAATTTTACTG
ENV1	7798 – 8036	Forward: 5′- CAGCAGGWAGCACKATGGG	239
		Reverse: 5′- GCARATGWGYTTTCCAGAGCADCC
ENV2	7950 – 8119	Forward: 5′- CTYCAGRCAAGARTCYTGGC	170
		Reverse: 5′- TCCCAYTSCAKCCARGTC
ENV3	8016 – 8299	Forward: 5′- TGCTCTGGAAARCWCATYTGC	284
		Reverse: 5′- AARCCTCCTACTATCATTATRA

^aSee Figure 1.

^bMixed nucleotides were present at some positions (W: A/T; K: G/T; R: A/G; Y: C/T; D: A/G/T; S: G/C).