Figure 3. Focal adhesion kinase (FAK) expression and phosphorylation are increased in human dermal fibroblasts (HDF) compared to human gingival fibroblasts (HGF).

(A) Cells were cultured until 80% confluence and incubated overnight in serum-free medium. Total protein was harvested, and subjected to Western blot analysis with the antibodies indicated. N = 3 average ±SE is shown * = p<0.05; ** = p<0.01. (B) Total RNA was harvested and subjected to real time PCR analysis with primers detecting FAK and 18S RNA. Data shown are the average value from three independent experiments (3 replicates per experiment) ±SE. * = p<0.05.