Figure 1.

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In vivo multimodal CARS imaging of a rats spinal cord. (a) In vivo imaging of a spinal cord with a MPO (left panel) and a 40× dipping objective lens (right panel). (b) and (c) In vivo CARS image of the superficial dorsal funiculus with (b) MPO and (c) 40× water immersion objective with 3.3 mm working distance. (d) In vivo CARS image of node of Ranvier. (e) XZ imaging by CARS (red), TPEF (green), and SFG (blue) reveals that optical penetration is unhindered by spinal cord meninges which allows imaging of myelin fibers ∼120 μm under the dura surface. The CARS signal diminishes at c.a. 30 μm from the surface of the white matter. Bar = 20 μm in all images. (Video 1, WMV, 18.3 MB)