Figure 2. MEF2 binding sites are critical for the promoter activity of PRL-3.
(A) The region between −158 and −33 is important for the transcriptional activity of the PRL-3 promoter, as measured by luciferase activity. (B) The effect of site-directed mutations in the MEF2 binding sites, as measured by luciferase activity. All luciferase activity were normalized to β-galactosidase activity and standardized to the normalized activity of pGL3-Basic. Each column represents the mean ± SD of three independent experiments. ** significantly different from the control at P<0.01.
