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. 2011 Nov 2;6(11):e27165. doi: 10.1371/journal.pone.0027165

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Xu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The effect of MEF2 plasmid on the luciferase activity of luc-158. Twenty-four hours after transfection, 293T cells were stimulated with 4-OHT for another 24 h and harvested for luciferase assay. (B) The constitutively active form of MEF2C up-regulated PRL-3-iso2 mRNA in several human cell lines. Cells were transfected with the indicated plasmids for 48 h and PRL-3 mRNA level was evaluated by real-time PCR.