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. 2011 Nov 2;6(11):e27360. doi: 10.1371/journal.pone.0027360

Figure 7. Lack of signs of apoptosis in mitotic supporting cells. Expression of supporting cell/epithelial markers in mitotic supporting cells.

Figure 7

(A-A″) In AdcD1-infected explants at 7 DIV, supporting cells with PH3+ (“PH3-Ser10 narrow antibody”, see text) mitotic profiles are negative for cleaved caspase-3. (B-B″) Double-staining shows that PH3+ mitotic cells lack expression of the supporting cell marker Sox9. (C-C″) Double-staining shows that PH3+ mitotic cells are positive for E-cadherin. (D-D″) Double-staining shows that PH3+ mitotic cells are positive for the supporting cell marker Sox2. Abbreviations: cD1, cyclin D1; PH3(na), phosphorylated histone H3-Ser10 narrow; casp, cleaved caspase-3; E-cad, E-cadherin. Scale bar, shown in D″: A–D″, 10 µm.