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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Curr Mol Med. 2011 Aug 1;11(6):503–511. doi: 10.2174/156652411796268740

Fig. (4). Confirmation of down regulated genes involved in cell proliferation, DNA repair, and metastasis in MB cells in response to DHA + VP16 compared to VP16 alone by real time PCR.

Fig. (4)

Total RNAs from Daoy and D283 Cells exposed to DHA, VP16 and their combination were used for cDNA synthesis, and it was then subject to be used for real time PCR using SYBR. The results were calculated using Pfaffl’s method [25-28], normalized using GAPDH and presented as relative expression level. The results shown were one representative of three independent experiments.