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. Author manuscript; available in PMC: 2011 Nov 2.
Published in final edited form as: Curr Mol Med. 2011 Aug 1;11(6):503–511. doi: 10.2174/156652411796268740

Fig. (5). Down regulation of CD44 and BCL2 in MB cells in response to DHA + VP16 compared to VP16 alone.

Fig. (5)

A). Total cellular proteins were prepared using lysis buffer containing 2% SDS, 100 mM DTT, 60 mM Tris (pH 6.8), 10% glycerol and separated on a 10% SDS-polyacrylamide gel, and transferred onto nitrocellulose membrane. Immunoblots were probed with antibodies specific for CD44, BCL2, caspase-3. The blots were then re-probed with β-actin as a loading control. B). Quantification of the results from A using ImageJ, expressed as a ratio of the expression level of target genes against β-actin. The results were calculated as mean ± SD. The statistical differences between the combination treatment vs. control or DHA or VP16 alone were determined using paired student’s t-test and performed using SPSS. ***p<0.001.