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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Microbiol. 2011 Mar 16;80(3):798–810. doi: 10.1111/j.1365-2958.2011.07609.x

Figure 7. RsbRA and RsbS are substrates for the RsbX phosphatase in unstressed cells.

Figure 7

Western blot analyses using anti-RsbRA (upper) or anti-RsbS (lower) antibody against IEF-separated crude cell extracts of unstressed strains bearing either a null allele in rsbU (U−; BSA70) or null alleles in both rsbU and rsbX (U−/X−; BSA151). Isoforms are 0 (unphosphorylated), 1 (singly phosphorylated) or 2 (doubly phosphorylated) for RsbRA; and 0 (unphosphorlylated) or 1 (singly phosphorylated) for RsbS.