In situ hybridization validation of thyroid bud enriched genes at E10.5. A, B. RIKEN cDNA 4930426D05 gene. C, D. Interferon inducible GTPase 1. E, F. Prolactin receptor. G, H. B-cell leukemia/lymphoma. I, J. Stanniocalcin 2, K, L. Sorbin and SH3 domain containing 2. M, N. Solute carrier family 16 (monocarboxylic acid transporters), member 2; Monocarboxylate transporter 8. O, P. T-box 3. Apart from strong accumulation in the thyroid bud prominent expression also is seen in the mesenchyme surrounding the lung bud. Q, R. Solute carrier family 4 (anion exchanger), member 4. S, T. Chemokine (C-X-C motif) ligand 12. Strong enrichment is seen in the thyroid bud as well as in the surrounding mesenchyme. Sagittal sections. Lung bud epithelium is outlined by dotted lines. Scale bar = 75 μm.