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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Jun 25;1820(3):417–426. doi: 10.1016/j.bbagen.2011.06.019

Figure 11.

Figure 11

ESI MS of a mixture of a recombinant human growth hormone protein/human transferrin (GHT) and the soluble part of transferrin receptor (TfR) shows facile formation of a 1:1 complex under near-physiological conditions (130 mM ammonium acetate 20mM ammonium bicarbonate, pH 7.4). Signal corresponding to unbound TfR reflects the excessive amount of the receptor in solution, while no signal of unbound GHT could be detected (the gray trace represents the reference mass spectrum of free GHT in solution in the absence of TfR). Reproduced with permission from [68].