Fig. 5.

Effects of various chemical inhibitors on SFN-induced up-regulation of HO-1 expression. BEAS-2B cells were treated with NAC (5 mM), Ly294002 (20 µM), and PD98059 (50 µM) for 1 hr prior to incubation with the indicated concentrations of SFN for 24 hr. Cell lysates were analyzed by Western blot analysis with anti-Nrf2 and anti-HO-1 antibodies. The blots were then stripped and re-probed with anti-β-actin antibody as a loading control. The normalized intensity of Nrf2 or HO-1 vs. β-actin is presented. Error bars represent the mean ± SEM for three independent experiments. ^*P < 0.05 compared to control.